Native MS dataset for: "Structural Insights into the Interaction Between Adenovirus 5C Hexon and Human Lactoferrin" ...
Native mass spectrometry dataset used in: Structural Insights into the Interaction Between Adenovirus 5C Hexon and Human Lactoferrin. Arun Dhillon, B. David Persson, Oleksandr Volkov, Hagen Sülzen, Alan Kádek, Petr Pompach, Sami Kereïche, Charlotte Uetrecht, Martin Lepšík, Niklas Arnberg and Sebasti...
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| Format: | Dataset |
| Language: | unknown |
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Zenodo
2024
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| Online Access: | https://dx.doi.org/10.5281/zenodo.10534069 https://zenodo.org/doi/10.5281/zenodo.10534069 |
| Summary: | Native mass spectrometry dataset used in: Structural Insights into the Interaction Between Adenovirus 5C Hexon and Human Lactoferrin. Arun Dhillon, B. David Persson, Oleksandr Volkov, Hagen Sülzen, Alan Kádek, Petr Pompach, Sami Kereïche, Charlotte Uetrecht, Martin Lepšík, Niklas Arnberg and Sebastian Zoll. The Journal of Virology (2024). doi: 10.1128/jvi.01576-23 Description: Native mass spectrometry (MS) analysis of the effect of solution ionic strength on the stoichiometry of interaction between human adenovirus HAdV-C5 hexon and human lactoferin. Sample processing: Prior to native MS measurements, hexon:Lf complex purified in buffer D was buffer exchanged into 20 mM aqueous ammonium acetate solution pH 7.0 (AA; ≥99.99% trace metals basis, Honeywell Research Chemicals) using two cycles of spin gel-filtration (MicroBioSpin P-6, Bio-Rad). The complex was subsequently diluted by 20 mM AA to 0.5 µM (estimated based on 3+3 stoichiometry). Alternatively, for low ionic strength conditions, the dilution was ... |
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