TN249-10 - Lipofuscin and Euphausiid Aging. Version 1.0

Age determination in crustaceans relies on quantification of a fluorescent age pigment, lipofuscin, that accumulates in eye stalks of krill over time. Lipofuscin includes a mixture of protein oxidation products formed in neural tissues. The extracted products are quantified using a fluorescence dete...

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Bibliographic Details
Main Authors: Harvey, H., Pleuthner, R.
Format: Dataset
Language:English
Published: UCAR/NCAR - Earth Observing Laboratory 2010
Subjects:
Online Access:https://dx.doi.org/10.5065/d69p2zpx
https://data.eol.ucar.edu/dataset/102.177
Description
Summary:Age determination in crustaceans relies on quantification of a fluorescent age pigment, lipofuscin, that accumulates in eye stalks of krill over time. Lipofuscin includes a mixture of protein oxidation products formed in neural tissues. The extracted products are quantified using a fluorescence detector. The concentration of lipofuscin is normalized to the protein content from the same tissues. The ratio of lipofuscin to protein concentration is the Lipofuscin Index and is calculated for each individual organism. In order for the index to reflect chronological age, a calibration is required using euphausiids of known age. A comparison of the calculated ages to the specific lengths for each krill provides the ages for those krill analyzed during each cruise. These data were collected aboard the UNOLS R/V Thompson ship. Data were collected during the Bering Sea Ecosystem Study-Bering Sea Integrated Ecosystem Research Program (BEST-BSIERP). BEST-BSIERP together are the Bering Sea project.