Data from: Capturing open ocean biodiversity: comparing environmental DNA metabarcoding to the continuous plankton recorder ...
Environmental DNA (eDNA) metabarcoding is emerging as a novel, objective tool for monitoring marine metazoan biodiversity. Zooplankton biodiversity in the vast and important open ocean is currently monitored through continuous plankton recorder (CPR) surveys, using ship-based bulk plankton sampling...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | English |
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Dryad
2020
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.w3r2280mq https://datadryad.org/stash/dataset/doi:10.5061/dryad.w3r2280mq |
| Summary: | Environmental DNA (eDNA) metabarcoding is emerging as a novel, objective tool for monitoring marine metazoan biodiversity. Zooplankton biodiversity in the vast and important open ocean is currently monitored through continuous plankton recorder (CPR) surveys, using ship-based bulk plankton sampling and morphological identification. We assessed whether eDNA metabarcoding (2 L filtered seawater) could capture similar Southern Ocean biodiversity as conventional CPR bulk sampling (~1500 L filtered seawater per CPR sample). We directly compared eDNA metabarcoding with (i) conventional morphological CPR sampling and (ii) bulk DNA metabarcoding of CPR collected plankton (two transects for each comparison, 40 and 44 paired samples respectively). A metazoan‐targeted cytochrome c oxidase I (COI) marker was used to characterize species-level diversity. In the 2 L eDNA samples this marker amplified large amounts of non‐metazoan picoplanktonic algae, but eDNA metabarcoding still detected up to 1.6 times more zooplankton ... : See materials and methods of Molecular Ecology paper "Capturing open ocean biodiversity: comparing environmental DNA metabarcoding to the continuous plankton recorder" ... |
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