Differential expression of olfactory genes in Atlantic salmon (Salmo salar) during the parr-smolt transformation ...
The anadromous salmon life-cycle includes two migratory events, downstream smolt migration and adult homing migration, during which they must navigate with high precision. During homing migration, olfactory cues are used for navigation in coastal and freshwater areas, and studies have suggested that...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2019
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.r7sqv9s7j https://datadryad.org/stash/dataset/doi:10.5061/dryad.r7sqv9s7j |
| Summary: | The anadromous salmon life-cycle includes two migratory events, downstream smolt migration and adult homing migration, during which they must navigate with high precision. During homing migration, olfactory cues are used for navigation in coastal and freshwater areas, and studies have suggested that the parr-smolt transformation has a sensitive period for imprinting. Accordingly, we hypothesized that there would be significant changes in gene expression in the olfactory epithelium specifically related to smoltification and sampled olfactory rosettes from hatchery-reared upper growth modal juvenile Atlantic salmon at three-week intervals from January to June, using lower growth modal non-smolting siblings as controls. A suite of olfactory receptors and receptor-specific proteins involved in functional aspects of olfaction and peripheral odor memorization was analyzed by qPCR. Gene expression in juveniles was compared with mature adult salmon of the same genetic strain caught in the river Gudenaa. All mRNAs ... : Olfactory epithelium from upper/lower mode salmon sampled during smoltification from January to June. RNA extracted and converted into cDNA. qPCR on olfactory receptors. ... |
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