Recent speciation and hybridization in Icelandic deep-sea isopods: An integrative approach using genomics and proteomics. ...
The crustacean marine isopod species Haploniscus bicuspis (G.O. Sars, 1877) shows circum-Icelandic distribution in a wide range of environmental conditions and along well-known geographic barriers, such as the Greenland-Iceland-Faroe (GIF) Ridge. We wanted to explore population genetics, phylogeogra...
| Main Authors: | , , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.r2280gbcd https://datadryad.org/stash/dataset/doi:10.5061/dryad.r2280gbcd |
| Summary: | The crustacean marine isopod species Haploniscus bicuspis (G.O. Sars, 1877) shows circum-Icelandic distribution in a wide range of environmental conditions and along well-known geographic barriers, such as the Greenland-Iceland-Faroe (GIF) Ridge. We wanted to explore population genetics, phylogeography and cryptic speciation as well as to investigate whether previously described, but unaccepted subspecies have any merit. Using the same set of specimens, we combined mitochondrial COI sequences, thousands of nuclear loci (ddRAD), and proteomic profiles, plus selected morphological characters using Confocal Laser Scanning Microscopy (CLSM). Five divergent genetic lineages were identified by COI and ddRAD, two south and three north of the GIF Ridge. Assignment of populations to the three northern lineages varied and detailed analyses revealed hybridization and gene flow between them, suggesting a single northern species with a complex phylogeographic history. No apparent hybridization was observed among lineages ... : All H. bicuspis specimens examined were sampled aboard the research vessels RV Meteor (M85/3), RV Poseidon (POS456) and RV Maria S. Merian (MSM75) during the IceAGE (2011), IceAGE2 (2013) and IceAGE_RR (2018) expeditions. The specimens were sorted at the laboratories of the DZMB (German Centre for Marine Biodiversity Research) and deposited in the collections of the Center of Natural History (Hamburg, Germany; see Supplementary Table S1). Specimens were collected using an epibenthic sledge or VanVeen grab. DNA extractions were performed using the mid-sections of the animals, leaving the cephalothorax and the pleon intact for subsequent morphological analyses and as partial vouchers in the collections. The animals were dissected carefully using a micro scissor and the gut was removed to avoid contaminations. If possible, the same animal was used for proteomics, COI barcoding and ddRAD, transferring half of the dissected tissue in a vial for genetics and the other half into a vial for proteomics. DNA was ... |
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