RNAScope analysis of human fetal tooth germ ...
This data was collected to verify the spatial localization of human fetal tooth germ cell types identified via sn-RNA-seq. Using this and other data, we have proposed a more precise developmental timeline and trajectory for human tooth development. ... : Description of methods used for collection/ge...
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| Format: | Dataset |
| Language: | English |
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Dryad
2022
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| Online Access: | https://dx.doi.org/10.5061/dryad.qnk98sfkk https://datadryad.org/stash/dataset/doi:10.5061/dryad.qnk98sfkk |
| Summary: | This data was collected to verify the spatial localization of human fetal tooth germ cell types identified via sn-RNA-seq. Using this and other data, we have proposed a more precise developmental timeline and trajectory for human tooth development. ... : Description of methods used for collection/generation of data: We used a RNAscope™ HiPlex12 Reagent Kit (488, 550, 650, 750) v2 Standard Assay (Advanced Cell Diagnostics, Inc.) and probes against 13 transcripts VWDE, SALL1, FGF4, IGFBP5, FGF10, PRRX1, FBN2, ENAM, PCDH7, SOX5, KRT5, and either DSPP or LGR6. Probe/Channel Key: Round 1 DAPI R1C1 VWDE R1C2 SALL1 R1C3 FGF4 R1C4 IGFBP5 R1C5 Round 2 DAPI R2C1 DSPP R2C2 LGR6 R2C2 FGF10 R2C3 PRRX1 R2C4 FBN2 R2C5 Round 3 DAPI R3C1 PCDH7 R3C3 SOX5 R3C4 KRT5 R3C5 Fresh frozen human fetal tooth germ tissue sections were stained according to the ACD protocol: https://acdbio.com/sites/default/files/UM%20324409%20RNAscope%20HiPlex%20v2%20User%20Manual%20%28488%2C%20550%2C%20650%2C750%29.pdf and imaged using a Nikon Ti2 with an Aura light engine and BrightLine Sedat filter set optimized for DAPI, FITC, TRITC, Cy5 & Cy7 (LED-DA/FI/TR/Cy5/Cy7-5X5M-A-000) for samples 1-4 or using a Yokogawa CSU-X1 spinning disk confocal microscope with a Celesta light engine and ORCA-Fusion ... |
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