Data from: Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells ...

• Main Authors: Matt, Ann-Sophie, Hu, Marian Y., Chang, William W.
• Format: Dataset
• Language: English
• Published: Dryad 2022
• Subjects: FOS: Natural sciences; Biomineralization; intracellular pH; metabolic CO2; Carbon fixation; Ocean acidification
• Online Access: https://dx.doi.org/10.5061/dryad.ns1rn8pwr; https://datadryad.org/stash/dataset/doi:10.5061/dryad.ns1rn8pwr

Many calcifying organisms utilize metabolic CO2 to generate CaCO3 minerals to harden their shells and skeletons. Carbonic anhydrases are evolutionary ancient enzymes that were proposed to play a key role in the calcification process with the underlying mechanisms being little understood. Here we used the calcifying primary mesenchyme cells of the sea urchin larva to study the role of cytosolic (iCAs) and extracellular carbonic anhydrases (eCAs) in the cellular carbon concentration mechanism (CCM). Molecular analyses identified iCAs and eCAs in PMCs and highlight the prominent expression of a GPI-anchored membrane-bound CA (Cara7). Intracellular pH recordings in combination with CO2 pulse experiments demonstrated iCA activity in PMCs. iCA activity measurements together with pharmacological approaches revealed an opposing contribution of iCAs and eCAs on the CCM. H+-selective electrodes were used to demonstrate eCA catalyzed CO2 hydration rates at the cell surface. Knock-down of Cara7 reduced extracellular CO2 ... : # Data from: Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells [https://doi.org/10.5061/dryad.ns1rn8pwr](https://doi.org/10.5061/dryad.ns1rn8pwr) ## Description of the data and file structure ### Figure 1 Phylogenetic analysis and expression patterns of iCAs and eCAs in the sea urchin larva. Expression of iCA Cara2 (C) and eCA Cara7 (D) along the first 72 hpf. ### Figure 2 Extracellular carbonic anhydrase activity is required for the calcification of the larval skeleton. (B) Biometric analyses of the post-oral rod after treatment with different concentrations of AZM and Dex-AZM. Asterisks indicate significant differences compared to controls with **p<0.001 (n = 3, One-Way ANOVA + Post-Hoc Test (Holm-Sidak)). Values are presented as mean ± SEM. (C) Determination of re-calcification rates by measuring the growth rate of the dissolved skeleton under pharmacological inhibition of CA activity by four concentrations (0 µM, 1 µM, 10 µM, 100 µM) of ...