Data for: Parallel recolonisations generate distinct genomic sectors in kelp following high magnitude earthquake disturbance ...
Large-scale disturbance events have the potential to drastically reshape biodiversity patterns. Notably, newly vacant habitat space cleared by disturbance can be colonised by multiple lineages, which can lead to the evolution of distinct spatial ‘sectors’ of genetic diversity within a species. We te...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.jdfn2z3bt https://datadryad.org/stash/dataset/doi:10.5061/dryad.jdfn2z3bt |
| Summary: | Large-scale disturbance events have the potential to drastically reshape biodiversity patterns. Notably, newly vacant habitat space cleared by disturbance can be colonised by multiple lineages, which can lead to the evolution of distinct spatial ‘sectors’ of genetic diversity within a species. We test for disturbance-driven sectoring of genetic diversity in intertidal southern bull kelp, Durvillaea antarctica (Chamisso) Hariot following the high-magnitude 1855 Wairarapa earthquake in New Zealand. Specifically, we use genotyping-by-sequencing (GBS) to analyse fine-scale population structure across the uplift zone to assess the fit of alternative recolonisaton models. Our analysis reveals that specimens from the uplift zone carry genomic signatures distinct from populations in other regions, consistent with recolonisation after the 1855 earthquake. Crucially, our analysis identifies two parapatric spatial-genomic sectors of D. antarctica at Turakirae Head, which experienced the most dramatic uplift. We infer ... : DNA was extracted and purified following the same method described in Peters et al. (2020), except that we used the updated Qiagen DNeasy Plant Pro DNA extraction kit. The kit protocol was followed, except that the lysis step was extended to 24 hours, and immediately after lysis, samples were treated with 100 µl isopropanol and incubated at 65°C for 30 minutes, vortexing every 15 minutes. Three new GBS libraries including 185 D. antarctica samples were used for this study (Supplementary Table 1; Appendix A). We also used GBS data for four samples sequenced within a previous study (Peters et al., 2020), bringing the total number of analysed individuals to 189 (Supplementary Table 1; Appendix A). DNA was digested using the PstI-HF enzyme, following the GBS protocol described by Elshire et al. (2011), with the same modifications described by Peters et al. (2020). The size selection varied between 200 – 500 bp and 200 – 600 bp (Supplementary Table 1). The four libraries were sequenced on four separate runs using ... |
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