Data from: Y-chromosome markers for the red fox ...
The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However...
| Main Authors: | , , , , , , , , |
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| Format: | Dataset |
| Language: | English |
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Dryad
2017
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.fq7dj https://datadryad.org/stash/dataset/doi:10.5061/dryad.fq7dj |
| Summary: | The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However, a megabase of red fox Y-chromosome sequence was recently identified over 2 scaffolds in the reference genome. Here, these scaffolds were scanned for repeated motifs, revealing 194 likely microsatellites. Twenty-three of these loci were selected for primer development and, after testing, produced a panel of 11 novel markers that were analyzed alongside 2 markers previously developed for the red fox from dog Y-chromosome sequence. The markers were genotyped in 76 male red foxes from 4 populations: 7 foxes from Newfoundland (eastern Canada), 12 from Maryland (eastern United States), and 9 from the island of Great Britain, as well as 48 foxes of known North American origin maintained on an experimental farm in ... : Raw Fragment Analysis DataThis is all of the raw data generated and analyzed for the development of a 13-microsatellite marker panel for the red fox Y-chromosome. It may be useful to researchers looking to calibrate calls made in new samples/populations using these markers. This zipped file includes all original .abi files, raw allele calls and peaks as produced with GeneMapper v3.5, a table of the final allele assigned to each individual at each marker, and the .ych file used to construct the haplotype network in Figure 2 with Network 5.0.0.0.Rando2017JHeredRawDataY-MarkersRedFox.zip ... |
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