Data from: Late Quaternary environmental and human impacts on the mitochondrial DNA diversity of four commensal rodents in Myanmar ...

• Main Author: Suzuki, Hitoshi
• Format: Dataset
• Language: English
• Published: Dryad 2020
• Subjects: Rattus exulans; Rattus rattus; Bandicota bengalensis; Mus musculus; cytb; Myanmar; Pacific
• Online Access: https://dx.doi.org/10.5061/dryad.7pvmcvdqm; https://datadryad.org/stash/dataset/doi:10.5061/dryad.7pvmcvdqm

We addressed the spatiotemporal characteristics of four commensal rodent species occurring in Myanmar in comparison with other areas of the Indo-Malayan region. We examined sequence variations of the mitochondrial cytochrome bgene (Cytb) in the Pacific rat (Rattus exulans), roof rat(Rattus rattuscomplex, RrC), lesser bandicoot rat (Bandicota bengalensis), and house mouse(Mus musculus) using the recently developed time-dependent evolutionary rates of mtDNA. The Cytbsequences of RrC from Myanmar were shown to belong to RrC Lineage II, and their level of genetic diversity was relatively high compared to those of the other three species. RrC was found to have experienced bottleneck and rapid expansion events at least twice in the late Pleistocene period in Myanmar and a nearby region. Accordingly, paleoclimatic environmental fluctuations were shown to be an important factor affecting rodents in the subtropics of the Indo-Malayan region. Our results show that human activities during the last 10,000 years of the ... : Sample collection in Myanmar was conducted mainly by staff members of the Department of Zoology, University of Yangon from 2013 to 2018. For this study, 35 individuals of Rattus exulans, 13 individuals of RrC, and 4 individuals ofM. musculus were collected and subjected to molecular analysis. DNA was extracted from the liver tissue from each individual using a Qiagen DNA Isolation kit (Hilden, Germany) following the manufacturer’s protocol. Sequence determination was performed for Cytb (1,034–1,140 bp) in accordance with a method described previously (Suzuki et al. 2015). The gene region was amplified through polymerase chain reaction (PCR) using the following protocol: 95°C for 10 min, followed by 30 cycles of 95°C for 30 s, 50°C for 30 s, and 60°C for 60 s, and finally, 72°C for 7 min. PCR products were sequenced using a PRISM Ready Reaction Dye DeoxyTerminator Cycle Sequencing kit Ver 3.1 and an ABI 3500 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). The nucleotide sequences reported in the ...