Microsatellite matrix of Poa annua ...
Comparative studies of invasive species in human-inhabited versus truly uninhabited habitats, particularly on their genetic structure, remain scarce. Sub-Antarctic islands provide an ideal system to study invasions in such contrasting environments as they represent semi-pristine conditions in highly...
| Main Authors: | , , , , , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2020
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.76hdr7st7 https://datadryad.org/stash/dataset/doi:10.5061/dryad.76hdr7st7 |
| Summary: | Comparative studies of invasive species in human-inhabited versus truly uninhabited habitats, particularly on their genetic structure, remain scarce. Sub-Antarctic islands provide an ideal system to study invasions in such contrasting environments as they represent semi-pristine conditions in highly remote areas that are accessible only through a small number of introduction routes. Here we studied the invasion genetics of annual bluegrass Poa annua on the Prince Edward Islands (PEI) that include the inhabited Marion Island and the uninhabited Prince Edward Island. We analysed variation at nuclear microsatellite loci and performed flow cytometry analyses to compare the genetic diversity and structure of this widespread invasive grass. We also used ecological niche models to estimate currently suitable habitat in these islands. We found high levels of genetic diversity and evidence for extensive admixture between genetically distinct groups of P. annua on Marion Island. In contrast, Prince Edward Island ... : Microsatellite-containing sequences were isolated by Ecogenics GmbH (Balgach, Switzerland). Size selected fragments from P. annua genomic DNA were enriched for nuclear microsatellite repeats using magnetic streptavidin beads and biotin-labelled tri- and tetra-mer repeat oligonucleotides. The microsatellite-enriched library was sequenced on a Roche 454 platform using the Roche GS FLX Titanium technology (Roche Diagnostics Corporation). This resulted in 861 reads containing microsatellite sequences with at least six tri- or tetra-nucleotide repeat units. Primers were designed for 24 of these loci, that showed both amplification and polymorphism. Of these, 12 loci were discarded due to amplification failure in most samples or poor electrophoretic profiles. Primers for the remaining 12 microsatellites are shown in Table S1. Genomic DNA was extracted from all samples using a modified cetyltrimethylammonium bromide (CTAB) method (Doyle & Doyle, 1987) with the addition of 0.2 M sodium sulphite to the extraction ... |
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