Plant DNA metabarcoding records from three cores of lacustrine lakes along a open larch forest-forest tundra-tundra transect at the Omoloy region, north-eastern Siberia ...
Here, we provide the raw plant DNA metabarcoding data archived in three Siberian lake sediment cores spanning the mid-Holocene to the present (7.6-0 cal ka BP), from northern typical tundra to southern open larch forest in the Omoloy region. There are three cores: 14-OM-20B, Lat. / °: 70.53, Lon. /...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2020
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.69p8cz900 https://datadryad.org/stash/dataset/doi:10.5061/dryad.69p8cz900 |
| Summary: | Here, we provide the raw plant DNA metabarcoding data archived in three Siberian lake sediment cores spanning the mid-Holocene to the present (7.6-0 cal ka BP), from northern typical tundra to southern open larch forest in the Omoloy region. There are three cores: 14-OM-20B, Lat. / °: 70.53, Lon. / °: 132.91, Ele. / m a.s.l.: 52, Modern vegetation: open larch forest, Lake area / km2: 0.26, Maximal depth / m: 3.4 14-OM-02B, Lat. / °: 70.72, Lon. / °: 132.67, Ele. / m a.s.l.: 58, Modern vegetation: forest tundra, Lake area / km2: 0.08, Maximal depth / m: 3.5 14-OM-12A, Lat. / °: 70.96, Lon. / °: 132.57, Ele. / m a.s.l.: 60, Modern vegetation: tundra, Lake area / km2: 0.09, Maximal depth / m: 4.5 Three lake sediment cores, 14OM12A (33 cm long), 14OM02B (49.5 cm long) and 14OM20B (86 cm long), were recovered from three sites using a UWITEC gravity corer (6 cm internal diameter) equipped with a hammer tool in July 2014. From the three cores, 16 bulk organic carbon samples were selected because of the lack of ... : Plant DNA was extracted from about 2–4 g of sediment using the PowerMax® Soil DNA Isolation Kit (MoBio Laboratories, Inc., USA) in a dedicated palaeogenetic laboratory in AWI. The pooled PCR products were sequenced on the Illumina HiSeq platform (2 x 125 bp, paired-end reads) at Fasteris SA sequencing service, Switzerland. To align, assign and filter Illumina sequences we used OBITools (Boyer et al., 2016). The raw sequecning data (fastq files), scripts for creating the taxonomic database and analyzing the raw sequencing data, tag-sample-matirx, taxonomic database are enclosed. ... |
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