Data from: Origins and functional diversification of salinity-responsive Na+, K+ ATPase α1 paralogs in salmonids ...

• Main Authors: Dalziel, Anne C., Bittman, Jesse, Mandic, Milica, Ou, Michelle, Schulte, Patricia M.
• Format: Dataset
• Language: English
• Published: Dryad 2014
• Subjects: Osmerus mordax; sodium potassium ATPase; Salmo salar; Coregonus clupeaformis; Esox lucius; Oncorhynchus mykiss; Salvelinus alpinus; Thymallus arcticus; Gene Structure and Function; Northern pike
• Online Access: https://dx.doi.org/10.5061/dryad.5c6v7; https://datadryad.org/stash/dataset/doi:10.5061/dryad.5c6v7

The Salmoniform whole-genome duplication is hypothesized to have facilitated the evolution of anadromy, but little is known about the contribution of paralogs from this event to the physiological performance traits required for anadromy, such as salinity tolerance. Here, we determined when two candidate salinity-responsive paralogs of the Na+, K+ ATPase α subunit (α1a and α1b) evolved and studied their evolutionary trajectories and tissue-specific expression patterns. We found that these paralogs arose during a small scale duplication event prior to the Salmoniform, but after the teleost, whole-genome duplication. The ‘freshwater paralog’ (α1a) is primarily expressed in the gills of Salmoniformes and an unduplicated freshwater sister-species (Esox lucius), and experienced positive selection in the fresh-water ancestor of Salmoniformes and Esociformes. Contrary to our predictions, the ‘saltwater paralog’ (α1b), which is more widely expressed than α1a, did not experience positive selection during the evolution ... : Key for Figure 2Accession numbers for all sequences used in Figure 2 and the short name used in sequence files for PAML and alignments (Appendix S1).Fig2_SequenceIDs_to_GenbankNumbers.txtFig4- Pike real time dataGene expression of Na+, K+ ATPase paralogs and 18S in the Northern Pike (Esox lucius)Fig4_Pike real time datal.xlsSupplementary Fig1Teleost Na+, K+ ATPase α1 (ATP1A1) gene tree reconstructed using Bayesian inference (BI) with MrBayes version 3.2.1 (Ronquist et al. 2012) and the GTR+I+Γ model of nucleotide evolution.SuppFig1_MrBayes_Jan_7_2013.rtfSupplementary Figure 2Teleost Na+, K+ ATPase α1 (ATP1A1) gene tree reconstructed using the maximum likelihood (ML) method with RAxML software version 7.2.7 (Stamatakis 2006) and the GTR+Γ model of nucleotide evolution.SuppFig2_RAxML_Dec_30.rtfTree file for PAMLTree file to be used with the one and free ratios models in PAML (Table 1, Test 1a).ATP1A1.treesControl file for PAML - Branch ModelControl file for PAML branch model to test for selection along Branch ...