Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours

We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest full...

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Bibliographic Details
Main Authors: Tanentzap, Andrew J., Vicari, Mark, Bazely, Dawn R.
Format: Dataset
Language:unknown
Published: Dryad Digital Repository 2014
Subjects:
symbioses
evolutionary ecology
plant defences
plant endophytes
salivary compounds
Sweden
Ontario
Festuca rubra
Epichloë festucae
Alces alces
Rangifer tarandus
Northern Sweden
Online Access:https://dx.doi.org/10.5061/dryad.06hn6/3
http://datadryad.org/resource/doi:10.5061/dryad.06hn6/3
Description
Summary:We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 8 hours over 2 days.

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