eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity ch...
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Online Access: | https://dx.doi.org/10.3929/ethz-b-000502270 http://hdl.handle.net/20.500.11850/502270 |
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ftdatacite:10.3929/ethz-b-000502270 2024-04-28T08:05:20+00:00 eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... Lacoursière‐Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K. Archambault, Philippe Deiner, Kristy Lodge, David M. Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis 2018 application/pdf https://dx.doi.org/10.3929/ethz-b-000502270 http://hdl.handle.net/20.500.11850/502270 en eng ETH Zurich info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 article-journal Text ScholarlyArticle Journal Article 2018 ftdatacite https://doi.org/10.3929/ethz-b-000502270 2024-04-02T12:34:54Z Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and ... : Ecology and Evolution, 8 (16) ... Article in Journal/Newspaper Arctic biodiversity Arctic Iqaluit DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
description |
Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and ... : Ecology and Evolution, 8 (16) ... |
format |
Article in Journal/Newspaper |
author |
Lacoursière‐Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K. Archambault, Philippe Deiner, Kristy Lodge, David M. Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis |
spellingShingle |
Lacoursière‐Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K. Archambault, Philippe Deiner, Kristy Lodge, David M. Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
author_facet |
Lacoursière‐Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K. Archambault, Philippe Deiner, Kristy Lodge, David M. Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis |
author_sort |
Lacoursière‐Roussel, Anaïs |
title |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
title_short |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
title_full |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
title_fullStr |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
title_full_unstemmed |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... |
title_sort |
edna metabarcoding as a new surveillance approach for coastal arctic biodiversity ... |
publisher |
ETH Zurich |
publishDate |
2018 |
url |
https://dx.doi.org/10.3929/ethz-b-000502270 http://hdl.handle.net/20.500.11850/502270 |
genre |
Arctic biodiversity Arctic Iqaluit |
genre_facet |
Arctic biodiversity Arctic Iqaluit |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.3929/ethz-b-000502270 |
_version_ |
1797575433872474112 |