eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...

Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity ch...

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Main Authors: Lacoursière‐Roussel, Anaïs, Howland, Kimberly, Normandeau, Eric, Grey, Erin K., Archambault, Philippe, Deiner, Kristy, Lodge, David M., Hernandez, Cecilia, Leduc, Noémie, Bernatchez, Louis
Format: Article in Journal/Newspaper
Language:English
Published: ETH Zurich 2018
Subjects:
Online Access:https://dx.doi.org/10.3929/ethz-b-000502270
http://hdl.handle.net/20.500.11850/502270
id ftdatacite:10.3929/ethz-b-000502270
record_format openpolar
spelling ftdatacite:10.3929/ethz-b-000502270 2024-04-28T08:05:20+00:00 eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ... Lacoursière‐Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K. Archambault, Philippe Deiner, Kristy Lodge, David M. Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis 2018 application/pdf https://dx.doi.org/10.3929/ethz-b-000502270 http://hdl.handle.net/20.500.11850/502270 en eng ETH Zurich info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 article-journal Text ScholarlyArticle Journal Article 2018 ftdatacite https://doi.org/10.3929/ethz-b-000502270 2024-04-02T12:34:54Z Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and ... : Ecology and Evolution, 8 (16) ... Article in Journal/Newspaper Arctic biodiversity Arctic Iqaluit DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language English
description Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and ... : Ecology and Evolution, 8 (16) ...
format Article in Journal/Newspaper
author Lacoursière‐Roussel, Anaïs
Howland, Kimberly
Normandeau, Eric
Grey, Erin K.
Archambault, Philippe
Deiner, Kristy
Lodge, David M.
Hernandez, Cecilia
Leduc, Noémie
Bernatchez, Louis
spellingShingle Lacoursière‐Roussel, Anaïs
Howland, Kimberly
Normandeau, Eric
Grey, Erin K.
Archambault, Philippe
Deiner, Kristy
Lodge, David M.
Hernandez, Cecilia
Leduc, Noémie
Bernatchez, Louis
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
author_facet Lacoursière‐Roussel, Anaïs
Howland, Kimberly
Normandeau, Eric
Grey, Erin K.
Archambault, Philippe
Deiner, Kristy
Lodge, David M.
Hernandez, Cecilia
Leduc, Noémie
Bernatchez, Louis
author_sort Lacoursière‐Roussel, Anaïs
title eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
title_short eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
title_full eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
title_fullStr eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
title_full_unstemmed eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity ...
title_sort edna metabarcoding as a new surveillance approach for coastal arctic biodiversity ...
publisher ETH Zurich
publishDate 2018
url https://dx.doi.org/10.3929/ethz-b-000502270
http://hdl.handle.net/20.500.11850/502270
genre Arctic biodiversity
Arctic
Iqaluit
genre_facet Arctic biodiversity
Arctic
Iqaluit
op_rights info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
cc-by-4.0
op_doi https://doi.org/10.3929/ethz-b-000502270
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