Recombinant mouse CRISP protein add-back experiments

I. Representative sperm from Crisp1 , and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa. Sperm were tethered...

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Bibliographic Details
Main Authors: Gaikwad, Avinash, O'Bryan, Moira
Format: Article in Journal/Newspaper
Language:unknown
Published: Monash University 2019
Subjects:
Molecular Biology
Genetics
FOS Biological sciences
Olympus
Orca
Online Access:https://dx.doi.org/10.26180/5dbf4bff1acce
https://bridges.monash.edu/articles/Recombinant_mouse_CRISP_protein_add-back_experiments/10246982
Description
Summary:I. Representative sperm from Crisp1 , and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa. Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps.

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