Physiology of psychrophilic bacteria from Antarctic sea ice ...

• Main Author: Nichols, David S
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Antarctic; Prydz Bay; Vestfold; Vestfold Hills; Antarc*; Antarctica; Sea ice
• Online Access: https://dx.doi.org/10.25959/23243252; https://figshare.utas.edu.au/articles/thesis/Physiology_of_psychrophilic_bacteria_from_Antarctic_sea_ice/23243252

Eighty seven psychrophilic bacterial strains, representing 85% of the total isolates, were isolated from eleven sea ice cores collected from the region of Prydz Bay near the Vestfold Hills, Eastern Antarctica during October-December 1993. The melt water of a further seven sea ice cores, which had been stored at 4°C until return to Australia, yielded only twelve psychrophilic strains (29% of total isolates). The storage of sea ice samples prior to bacterial isolation therefore appears to effect the survival and/or viability of psychrophilic isolates. Chemotaxonomic investigation of selected strains, based on cellular fatty acid composition, identified isolates of the Genera Flavobacterium-Cytophaga, Vibrio, Shewanella and Psychrobacter. Two chemotaxonomic groups of Shewanella sp. were found to contain eicosapentaenoic acid [20:5(03; EPA] while one group of Vibrio sp. produced docosahexaenoic acid [22:6(.03; DHA]. The temperature-growth characteristics of three psychrophilic strains (ACAM 456, JS6P2OrZB, ...