Investigation into the microbiological causes of epizootics of Pacific Oyster larvae (Crassostrea gigas) in commercial production ...

An investigation was undertaken into the cause of Pacific oyster (Crassostrea gigas) larvae epizootics occurring in a commercial hatchery located at Bicheno, Tasmania. An extensive monitoring survey was conducted at the hatchery to characterise the microbiological environment in the immediate vicini...

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Bibliographic Details
Main Author: Chapman, CC
Format: Thesis
Language:unknown
Published: University Of Tasmania 2023
Subjects:
Online Access:https://dx.doi.org/10.25959/23206049.v1
https://figshare.utas.edu.au/articles/thesis/Investigation_into_the_microbiological_causes_of_epizootics_of_Pacific_Oyster_larvae_Crassostrea_gigas_in_commercial_production/23206049/1
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Summary:An investigation was undertaken into the cause of Pacific oyster (Crassostrea gigas) larvae epizootics occurring in a commercial hatchery located at Bicheno, Tasmania. An extensive monitoring survey was conducted at the hatchery to characterise the microbiological environment in the immediate vicinity of the larvae associated with different production outcomes. The surveys were performed over a 12-month period and included eight different production runs. Seven of the eight production runs terminated in disease incidence with larvae exhibiting disease symptoms consistent with bacterial infection previously described as bacillary necrosis, caused primarily by pathogenic Vibrio spp. Using production data records, physiochemical data, dissolved nutrient analysis, bacterial cultivation, TRFLP fingerprinting and 16S rRNA gene clone library analysis, two separate investigations were undertaken. In the first investigation microbial communities in each compartment of the larvae tank (water column, larvae, biofilm) ...