The role of ligand-gated chloride channels in behavioural alterations at elevated CO2 in a cephalopod ...
Two-toned pygmy squid (Idiosepius pygmaues) were collected from the wild and after 1-6 days of acclimation at ambient CO 2 levels were exposed to ambient (~450 µatm) or elevated (~1,000 µatm) CO 2 levels for 7 days. Squid were then treated with sham, 10.86 µM gabazine (...
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| Language: | unknown |
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James Cook University
2021
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| Online Access: | https://dx.doi.org/10.25903/y6kz-hm11 https://researchdata.jcu.edu.au/published/3a248d4048d817dcf358b9d61b318a80 |
| Summary: | Two-toned pygmy squid (Idiosepius pygmaues) were collected from the wild and after 1-6 days of acclimation at ambient CO 2 levels were exposed to ambient (~450 µatm) or elevated (~1,000 µatm) CO 2 levels for 7 days. Squid were then treated with sham, 10.86 µM gabazine (GABA-A R antagonist) or 100 µM picrotoxin (non-specific GABA-A R antagonist) by bath application for 30 minutes, followed by a behavioural test; squid were placed in a square tank with a mirror along the length of one wall. Video recordings of each behavioural test were taken for approx. 16 minutes (introduction to the tank + 15 minute behavioural trial + tank approach). The behavioural videos were manually analysed for: • Proportion of squid that touched the mirror softly/aggressively<br />• Latency to the first soft/aggressive mirror touch (only for squid that touched the mirror)<br />• ... |
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