Atlantic Puffin (Fratercula arctica) single-nucleotide polymorphism (SNP) datasets ...

• Main Authors: Carvey, Quinn, Pavey, Scott A., Diamond, Antony W., Davoren, Gail K., Lavoie, Raphael A., Legard, Matthew, Robertson, Gregory J., Runnells, Emily, Petalas, Christina, Major, Heather L.
• Format: Dataset
• Language: unknown
• Published: UNB 2024
• Subjects: Canada; Atlantic puffin; fratercula; Fratercula arctica
• Online Access: https://dx.doi.org/10.25545/xwgrbp; https://dataverse.lib.unb.ca/citation?persistentId=doi:10.25545/XWGRBP

Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Samples were sequenced on one lane using 150 bp, paired-end sequencing with an Illumina NovaSeq 6000. Data were assembled with Stacks v.2.6.1, see publication for full methodological details. Filtered SNP datasets used in genetic analyses are included here. ...