Atlantic Puffin (Fratercula arctica) single-nucleotide polymorphism (SNP) datasets ...
Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Sa...
Main Authors: | , , , , , , , , , |
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Format: | Dataset |
Language: | unknown |
Published: |
UNB
2024
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Subjects: | |
Online Access: | https://dx.doi.org/10.25545/xwgrbp https://dataverse.lib.unb.ca/citation?persistentId=doi:10.25545/XWGRBP |
Summary: | Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Samples were sequenced on one lane using 150 bp, paired-end sequencing with an Illumina NovaSeq 6000. Data were assembled with Stacks v.2.6.1, see publication for full methodological details. Filtered SNP datasets used in genetic analyses are included here. ... |
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