MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS.
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| Format: | Dataset |
| Language: | English |
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MassIVE
2019
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| Online Access: | https://dx.doi.org/10.25345/c5594p https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000084153 |
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ftdatacite:10.25345/c5594p |
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openpolar |
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ftdatacite:10.25345/c5594p 2023-05-15T16:05:35+02:00 MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. Khudyakov, Jane 2019 https://dx.doi.org/10.25345/c5594p https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000084153 en eng MassIVE Mass Spectrometry dataset Dataset 2019 ftdatacite https://doi.org/10.25345/c5594p 2021-11-05T12:55:41Z Dataset Elephant Seals DataCite Metadata Store (German National Library of Science and Technology) |
| institution |
Open Polar |
| collection |
DataCite Metadata Store (German National Library of Science and Technology) |
| op_collection_id |
ftdatacite |
| language |
English |
| format |
Dataset |
| author |
Khudyakov, Jane |
| spellingShingle |
Khudyakov, Jane MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| author_facet |
Khudyakov, Jane |
| author_sort |
Khudyakov, Jane |
| title |
MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| title_short |
MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| title_full |
MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| title_fullStr |
MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| title_full_unstemmed |
MassIVE MSV000084153 - Blubber proteome response to repeated ACTH administration in juvenile elephant seals : Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS. |
| title_sort |
massive msv000084153 - blubber proteome response to repeated acth administration in juvenile elephant seals : proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated acth administration experiment (for details, see mccormley et al., 2018, doi: 10.1093/conphys/coy040; deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). sample preparation was conducted using the qiazol method as previously described (khudyakov et al., 2018, doi:10.1242/bio.036731). four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth acth administration) were labeled with 8-plex itraq, fractionated off-line, pooled into 4 fractions, and analyzed by hplc-ms/ms. |
| publisher |
MassIVE |
| publishDate |
2019 |
| url |
https://dx.doi.org/10.25345/c5594p https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000084153 |
| genre |
Elephant Seals |
| genre_facet |
Elephant Seals |
| op_doi |
https://doi.org/10.25345/c5594p |
| _version_ |
1766401475635838976 |