lip7-3 (ctrl) ...

Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm,...

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Bibliographic Details
Main Author: Nicolas, William
Format: Dataset
Language:English
Published: CaltechDATA 2020
Subjects:
Escherichia coli
PK2, BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
artica
Online Access:https://dx.doi.org/10.22002/67dvj-zbx16
https://data.caltech.edu/doi/10.22002/67dvj-zbx16
id ftdatacite:10.22002/67dvj-zbx16
record_format openpolar
spelling ftdatacite:10.22002/67dvj-zbx16 2023-07-23T04:18:13+02:00 lip7-3 (ctrl) ... Nicolas, William 2020 mp4 jpg mrc rec https://dx.doi.org/10.22002/67dvj-zbx16 https://data.caltech.edu/doi/10.22002/67dvj-zbx16 en eng CaltechDATA Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode cc-by-nc-4.0 Escherichia coli PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor dataset Dataset 2020 ftdatacite https://doi.org/10.22002/67dvj-zbx16 2023-07-03T17:25:32Z Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and further purification by . ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing through Potter and sonicator.. Sample Preparation: Fiducials were prepared my method (see lab archive). ... Dataset artica DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language English
topic Escherichia coli
PK2, BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
spellingShingle Escherichia coli
PK2, BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
Nicolas, William
lip7-3 (ctrl) ...
topic_facet Escherichia coli
PK2, BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
description Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and further purification by . ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing through Potter and sonicator.. Sample Preparation: Fiducials were prepared my method (see lab archive). ...
format Dataset
author Nicolas, William
author_facet Nicolas, William
author_sort Nicolas, William
title lip7-3 (ctrl) ...
title_short lip7-3 (ctrl) ...
title_full lip7-3 (ctrl) ...
title_fullStr lip7-3 (ctrl) ...
title_full_unstemmed lip7-3 (ctrl) ...
title_sort lip7-3 (ctrl) ...
publisher CaltechDATA
publishDate 2020
url https://dx.doi.org/10.22002/67dvj-zbx16
https://data.caltech.edu/doi/10.22002/67dvj-zbx16
genre artica
genre_facet artica
op_rights Creative Commons Attribution Non Commercial 4.0 International
https://creativecommons.org/licenses/by-nc/4.0/legalcode
cc-by-nc-4.0
op_doi https://doi.org/10.22002/67dvj-zbx16
_version_ 1772180432119398400

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