lip7-3 (ctrl) ...
Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm,...
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Format: | Dataset |
Language: | English |
Published: |
CaltechDATA
2020
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Subjects: | |
Online Access: | https://dx.doi.org/10.22002/67dvj-zbx16 https://data.caltech.edu/doi/10.22002/67dvj-zbx16 |
Summary: | Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and further purification by . ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing through Potter and sonicator.. Sample Preparation: Fiducials were prepared my method (see lab archive). ... |
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