Asexual reproduction and statoliths formation of Cotylorhiza tuberculata under predicted future (2100) temperature and ocean acidification conditions
The database provides laboratory data obtained from two experiments carried out during 2019 using polyps of the jellyfish Cotylorhiza tuberculata. The experiments considered current and ca. 2100 winter (Experiment 1) and summer (Experiment 2) conditions under the RCP8.5 scenario (IPCC, 2013). Experi...
| Main Authors: | , , , |
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| Format: | Other/Unknown Material |
| Language: | English |
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DIGITAL.CSIC
2020
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.20350/digitalcsic/12662 https://digital.csic.es/handle/10261/221219 |
| Summary: | The database provides laboratory data obtained from two experiments carried out during 2019 using polyps of the jellyfish Cotylorhiza tuberculata. The experiments considered current and ca. 2100 winter (Experiment 1) and summer (Experiment 2) conditions under the RCP8.5 scenario (IPCC, 2013). Experiment 1 conditions were 18ºC and two pH levels (current: 7.9 and, reduced: 7.7): and experiment 2 conditions were 24ºC and 30ºCand two pH levels (current: 8.0 and reduced: 7.7). The database contains 3 datasets: 1) Survival and asexual reproduction: Data of the survival and asexual reproduction of the polyps collected during the described experimental conditions. This first dataset includes the number of polyps, buds and polyps undergoing budding, and the number of ephyrae released by strobilation of polyps from the different temperature and pCO2 treatments. Each experiment last for 36 days and the number of replicates per treatment was 6 for experiment 1 and 3 for experiment 2. Seawater salinity was 38. AT was determined by potentiometric titration using a Metrohm 794 Titroprocessor and Fixanal (0.5 mol l-1 of HCl) as titrant (Mintrop et al. 2000). pH was measured spectrophotometrically (Clayton and Byrne 1993) using m-cresol purple as indicator, and consequently, values were expressed in total scale. pCO2 was derived from pH, AT nitrate and silicate using CO2SYS software (Lewis and Wallace 1998). 2) Ephyrae Measurements: This dataset includes measurements of the ephyrae released from the mentioned experimental conditions measured under optical microscopy. The total body diameter of the ephyrae (long axis and short axis, BDL and BDW) and the total marginal lappet length and width were measured at 5x magnification. Rhopalia and statocyst (crystal conglomeration) length and width were measured at 40x magnification. 3) Statolith Measurements: Includes the number of statoliths in each rhopalium. For each ephyra, the length (long axis) and width (short axis) of all statoliths in all rhopalia were measured on a computer screen using AxioVisionLE software at 100x magnification. The volume of each statolith was indirectly calculated from measurements of length and width, considering the statolith shape as a hexagonal prism. : This data set includes data collected in 2019 from laboratory experiments using polyps of the jellyfish Cotylorhiza tuberculata exposed to current and future seawater pH and temperature conditions. The dataset includes information of the survival and asexual reproduction of polyps under different experimental treatments as well as, data of the measurements of ephyrae and statoliths. : The database is composed by 3 datasest: 1) Survival and asexual reproduction 2) Ephyrae Measurements 3) Statolith Measurements |
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