Label-free and fluorescent imaging data of microparticle association with Salmon Keratocytes ...

The dataset contains multimodal and multidimensional microscopy images of the interactions between different microparticles either in skin keratocytes cells (SKC) or in corneal keratocyte cells (CKC) of Atlantic Salmon. All images were captured at room temperature, and the dataset includes both test...

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Bibliographic Details
Main Authors: Thiyagarajan, Dhivya B, Afzal, Bilal M, Wolfson, Deanna
Format: Dataset
Language:unknown
Published: DataverseNO 2024
Subjects:
Online Access:https://dx.doi.org/10.18710/kbeqt9
https://dataverse.no/citation?persistentId=doi:10.18710/KBEQT9
Description
Summary:The dataset contains multimodal and multidimensional microscopy images of the interactions between different microparticles either in skin keratocytes cells (SKC) or in corneal keratocyte cells (CKC) of Atlantic Salmon. All images were captured at room temperature, and the dataset includes both test (with microparticles) and control (without microparticles) conditions. These interactions were captured using two different microscopes: 1) 3D Cell Explorer-fluo (Nanolive): a holotomographic imaging method which produces label-free 3D images using refractive index as the contrast mechanism and can optionally also provide 2D correlative fluorescence images. 2) DeltaVision Elite (GE Healthcare): a widefield fluorescence microscope with integrated deconvolution for improved 3D imaging, which can provide correlative 2D differential interference contrast (DIC) images. The main goal of this study was to understand how different microparticles interact with SKC and CKC, which are two of the important defense cells for ...