Tissue damage and tissue localisation of the bacteria Vibrio aestuarianus during an experimental infection (oyster Crassostrea gigas) ...

• Main Authors: Parizadeh, Leila, Travers, Marie-Agnes, Garcia, Celine, Chollet, Bruno
• Format: Dataset
• Language: unknown
• Published: SEANOE 2018
• Subjects: Pathogenesis; Vibrio; Localisation; Damages; Oyster; Bacteria; Tissue; Experimentally infected; Crassostrea gigas
• Online Access: https://dx.doi.org/10.17882/61299; https://www.seanoe.org/data/00501/61299/

Histopathological damage and localisation of Vibrio aestuarianus in diverse oyster tissues were studied on experimentally infected animals by histology and immunohistochemistry with a polyclonal antibody. Histological slides were scaned and are here presented. On day 1, V. aestuarianus was detected as few foci in the gills of 5 out of 14 animals. Nevertheless, as early as this first day after bacterial contact, first signs of histological damage were observed. These lesions could be classified into three classes: (type 1) lysis of the sub-epithelial connective tissue in the mantle, (type 2) epithelial atrophy of digestive diverticula with dilation of the diverticulum lumen and (type 3) haemocyte lysis and/or infiltration by haemocytes and/or haemocyte agglutination in haemolymph vessels. In oysters immersed in bacterial bath and sampled on day 4, intense colonization of all oyster tissues was observed. To be precise, stained bacteria-like cells were detected (i) in gills, as attached to epithelial cells or ...