Tissue damage and tissue localisation of the bacteria Vibrio aestuarianus during an experimental infection (oyster Crassostrea gigas) ...

Histopathological damage and localisation of Vibrio aestuarianus in diverse oyster tissues were studied on experimentally infected animals by histology and immunohistochemistry with a polyclonal antibody. Histological slides were scaned and are here presented. On day 1, V. aestuarianus was detected...

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Bibliographic Details
Main Authors: Parizadeh, Leila, Travers, Marie-Agnes, Garcia, Celine, Chollet, Bruno
Format: Dataset
Language:unknown
Published: SEANOE 2018
Subjects:
Online Access:https://dx.doi.org/10.17882/61299
https://www.seanoe.org/data/00501/61299/
Description
Summary:Histopathological damage and localisation of Vibrio aestuarianus in diverse oyster tissues were studied on experimentally infected animals by histology and immunohistochemistry with a polyclonal antibody. Histological slides were scaned and are here presented. On day 1, V. aestuarianus was detected as few foci in the gills of 5 out of 14 animals. Nevertheless, as early as this first day after bacterial contact, first signs of histological damage were observed. These lesions could be classified into three classes: (type 1) lysis of the sub-epithelial connective tissue in the mantle, (type 2) epithelial atrophy of digestive diverticula with dilation of the diverticulum lumen and (type 3) haemocyte lysis and/or infiltration by haemocytes and/or haemocyte agglutination in haemolymph vessels. In oysters immersed in bacterial bath and sampled on day 4, intense colonization of all oyster tissues was observed. To be precise, stained bacteria-like cells were detected (i) in gills, as attached to epithelial cells or ...