A new high throughput sequencing assay for characterising the diversity of natural Vibrio communities and its application to a Pacific oyster mortality event - data analysis workflow
A new high throughput sequencing approach targeting the heat shock protein (hsp60) as a phylogenetic marker was developed to more precisely discriminate members of the Vibrio genus in environmental samples. There is a need for an accurate high throughput assay for defining Vibrio community compositi...
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| Format: | Text |
| Language: | unknown |
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Open Science Framework
2021
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| Online Access: | https://dx.doi.org/10.17605/osf.io/yxjtu https://osf.io/yxjtu/ |
| Summary: | A new high throughput sequencing approach targeting the heat shock protein (hsp60) as a phylogenetic marker was developed to more precisely discriminate members of the Vibrio genus in environmental samples. There is a need for an accurate high throughput assay for defining Vibrio community composition in natural samples and this Vibrio-centric hsp60 sequencing assay offers the potential for precise high throughput characterisation of Vibrio diversity, providing an enhanced platform for dissecting Vibrio dynamics in the environment. Associated publication DOI: 10.3389/fmicb.2019.02907 |
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