A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen ...

• Main Authors: Kubickova, Barbara, Schenk, Jörg A., Ramm, Franziska, Markuškienė, Kornelija, Reetz, Jochen, Dremsek, Paul, Tamosiunas, Paulius Lukas, Cepulyte, Laima, Trinh, Hoai Anh, Scholz, Johannes, Memczak, Henry, Hovestädt, Marc, Ryll, René, Petraityte-Burneikiene, Rasa, Corman, Victor M., Andersson, Anika, Becher, Dietmar, Groschup, Martin H., Kubick, Stefan, Sellrie, Frank, Johne, Reimar, Ulrich, Rainer G.
• Format: Text
• Language: unknown
• Published: Freie Universität Berlin 2021
• Subjects: Hepatitis E virus; HEV-1; HEV-2; HEV-3; HEV-4; HEV-7; ratHEV; batHEV; cvHEV; Monoclonal antibody; Cross-reactivity; Cell-free synthesis; 600 Technik, Medizin, angewandte Wissenschaften610 Medizin und Gesundheit615 Pharmakologie, Therapeutik; Common vole
• Online Access: https://dx.doi.org/10.17169/refubium-38294; https://refubium.fu-berlin.de/handle/fub188/38578

To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli–expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli–expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and Spodoptera frugiperda (Sf21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of ...