Temperature responses of different functional traits in Arctic phytoplankton ...

• Main Authors: Rehder, Linda, Rokitta, Sebastian D, Buschmann, Isabelle, Jasper, Levke, Hoppe, Clara Jule Marie, Rost, Björn
• Format: Dataset
• Language: unknown
• Published: PANGAEA 2024
• Subjects: Diatom; growth; ice algae; Phaeocystis pouchetii; physiology; Phytoplankton; Temperate; Experiment; Sampling date; Sample ID; Type of study; Laboratory; Species, unique identification; Species, unique identification URI; Strain; Time in years; Location; Treatment: temperature; Treatment: partial pressure of carbon dioxide; Treatment: light:dark cycle; Treatment: light intensity; Treatment: salinity; Medium; Generation; Growth rate; Carbon, organic, particulate, per cell; Nitrogen, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Chlorophyll a per cell; Chlorophyll a/particulate organic carbon ratio; Carbon, organic, particulate, net production, per cell; Absorption cross section of PSII photochemistry; Time constant for the initial part of an single turnover relaxation phase; Quantum yield efficiency of photosystem II; Electron transport rate, absolute; Light use efficiency; Light saturation; Maximal electron transport rate; Daily cell counting, Beckmann coulter Counter; Flash combustions technique, CN Analyzer, EuroVector EA 3000; Calculated; Fluorometry; Fast repetition rate fluorometry FRRF; Arctic; Coulter; Kongsfjord; Svalbard; Fram Strait; Kongsfjord*
• Online Access: https://dx.doi.org/10.1594/pangaea.968670; https://doi.pangaea.de/10.1594/PANGAEA.968670

This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), ... : Thalassiosira hylina strain KB3SS5, isolated 2020 in Kongsjford (Svalbard)Micromonas pusilla strain CLP1, isolated 2014 in Kongsfjord (Svalbard)Nitzschia frigida strain UIO-542, isolated in 2019 in the Fram strait ...