Seawater carbonate chemistry and Phaeocystis pouchetii's responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) ...

• Main Authors: Rokitta, Sebastian D, Großmann, Christian H, Werner, Elisa, Moye, Jannika, Castellani, Giulia, Nöthig, Eva-Maria, Rost, Björn
• Format: Dataset
• Language: unknown
• Published: PANGAEA 2024
• Subjects: Arctic; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Haptophyta; Laboratory experiment; Light; Open ocean; Pelagos; Phaeocystis pouchetii; Phytoplankton; Polar; Primary production/Photosynthesis; Single species; Temperature; Type of study; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Experimental treatment; Date/time start; Date/time end; Strain; Replicate; Treatment temperature; Treatment light intensity; Treatment partial pressure of carbon dioxide; Carbon, inorganic, dissolved; pH; Temperature, water; Alkalinity, total; Partial pressure of carbon dioxide water at sea surface temperature wet air; Growth rate; Carbon, organic, particulate, per cell; Nitrogen, organic, particulate, per cell; Carbon, organic, particulate, production per cell; Production of particulate organic nitrogen; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Chlorophyll a per cell; Chlorophyll a/particulate organic carbon ratio; Electron transport rate, relative; Light acclimation index; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Maximum photosynthesis rate, oxygen, per chlorophyll a; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Alfred Wegener Institute
• Online Access: https://dx.doi.org/10.1594/pangaea.964944; https://doi.pangaea.de/10.1594/PANGAEA.964944

We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and ...