Flow cytometry dataset from first year sea ice (FYI) core bottom 5 cm sections showing the abundance of microorganisms (< 20 µm) during leg 2, 3 (February, March and April 2020) of the Arctic MOSAiC expedition ...

• Main Authors: Müller, Oliver, Fong, Allison A, Olsen, Lasse Mørk, Hoppe, Clara Jule Marie, Torstensson, Anders, Heitman, Laura, Petelenz, Elzbieta, Larsen, Aud, Tsagaraki, Tatiana M, Mayers, Kyle, Langvad, Maria, Granskog, Mats A, Bratbak, Gunnar
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: flow cytometry; Ice core; microbial abundance; MOSAiC_ECO; MOSAiC expedition; Event label; Sample code/label; Leg Number; Activity description; DATE/TIME; Device type; Depth, bathymetric; LATITUDE; LONGITUDE; Feature; Identification; DEPTH, ice/snow; Core length; Volume; Sample volume; Factor; Red only fluorescing picophytoplankton; Red only fluorescing nanophytoplankton; Red only fluorescing nanophytoplankton, small; Red only fluorescing nanophytoplankton, large; Orange and red fluorescing picophytoplankton; Orange and red fluorescing nanophytoplankton; Orange fluorescing prokaryote picophytoplankton; Heterotrophic nanophytoplankton; Heterotrophic prokaryotes; Heterotrophic prokaryotes with relatively low nucleic acid; Heterotrophic prokaryotes with relatively high nucleic acid; Heterotrophic prokaryotes, medium; Heterotrophic prokaryotes, large; Heterotrophic prokaryotes, very large; Virus-like particles; Virus, low DNA fluorescence; Virus, medium DNA fluorescence; Virus, high DNA fluorescence; Ice corer; Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm blue laser]; Flow cytometry system, Becton Dickinson, FACSCalibur; PS122/2; PS122/3; Polarstern; Multidisciplinary drifting Observatory for the Study of Arctic Climate MOSAiC; Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean HAVOC; Arctic; Arctic Ocean; ice core; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.963560; https://doi.pangaea.de/10.1594/PANGAEA.963560

This dataset is a subset of the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC) sampled from first year sea ice (FYI) core bottom 5 cm sections from leg 2 and 3 (February, March, April 2020). For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied (details provided in the data-file), to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., to be submitted to Elementa). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021).Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). ...