Seawater carbonate chemistry and cellular metabolism of the great scallop Pecten maximus ...

• Main Authors: Götze, Sandra, Bock, Christian, Eymann, Charlotte, Lannig, Gisela, Steffen, Jennifer B M
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Other studied parameter or process; Oxygen; Pecten maximus; Single species; Temperate; Temperature; Type of study; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Temperature, technical; Individuals; Survival; Identification; Sample type; Uridine diphosphate glucose; Triphosphates; Succinate; Valine; Leucine; Isoleucine; Lysine; Arginine; Glutamine; Homocysteine; Glutamate; Alanine; beta-Alanine; Aspartate; Glycine; Sarcosine; Threonine; Taurine; Hypotaurine; Betaine; sn-Glycero-3-phosphocholine; Choline; O-Phosphocholine; Trimethylamine N-oxide; Trimethylamine; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.959915; https://doi.pangaea.de/10.1594/PANGAEA.959915

In the ocean the main climate drivers affecting marine organisms are warming, hypercapnia, and hypoxia. We investigated the acute effects of warming (W), warming plus hypercapnia (WHc, ~1800 μatm CO2), warming plus hypoxia (WHo, ~12.1 kPa O2), and a combined exposure of all three drivers (Deadly Trio, DT) on king scallops (Pecten maximus). All exposures started at 14 °C and temperature was increased by 2 °C once every 48 h until the lethal temperature was reached (28 °C). Gill samples were taken at 14 °C, 18 °C, 22 °C, and 26 °C and analyzed for their metabolic response by 1H-nuclear magnetic resonance (NMR) spectroscopy. Scallops were most tolerant to WHc and most susceptible to oxygen reduction (WHo and DT). In particular under DT, scallops' mitochondrial energy metabolism was affected. Changes became apparent at 22 °C and 26 °C involving significant accumulation of glycogenic amino acids (e.g. glycine and valine) and anaerobic end-products (e.g. acetic acid and succinate). In line with these observations ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-06-20. ...