Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates ...

• Main Authors: Bock, Christian, Wermter, Felizitas Charlotte, Schalkhausser, Burgel, Blicher, Martin E, Pörtner, Hans-Otto, Lannig, Gisela, Sejr, Mikael K
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Chlamys islandica; Coast and continental shelf; Laboratory experiment; Mollusca; North Atlantic; Other studied parameter or process; Pecten maximus; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Temperature, water; Treatment; Number; Number of claps; Number of claps, standard deviation; Phosphate, inorganic/phospho-L-arginine ratio; Phosphate, inorganic/phospho-L-arginine ratio, standard deviation; Phospho-L-arginine; pH; pH, standard deviation; Exponential rate constant for recovery; Exponential rate constant for recovery, standard deviation; Maximal surplus oxidative flux, adenosine triphosphate per time; Maximal surplus oxidative flux, adenosine triphosphate per time, standard deviation; Halftime of recovery; Temperature, water, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Salinity; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.959731; https://doi.pangaea.de/10.1594/PANGAEA.959731

Object：Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods： Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-06-08. ...