Plant DNA taxa detected in Lake Savine sediments (North-western Alps) over the last 8000 years ...

• Main Authors: Giguet-Covex, Charline, Chen, Wentao, Ficetola, Francesco Gentile, Gielly, Ludovic, Pansu, Johan
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Alpes; Lake sediment core; Landscape; plant taxa; Sedimentary DNA; AGE; Alchemilla; Apiaceae; Asteraceae; Asteroideae; Bartsia alpina; Betulaceae; Boraginaceae; Brassicaceae; Campanulaceae; Cardamineae; Carex; Caryophyllaceae; Cerastium cerastoides; Colurieae; Cupressaceae; Euphrasia; Mentheae; Myosotis; Onagraceae; Oxyria digyna; Plantago; Poeae; Potentilla; Primulaceae; Ranunculaceae; Ranunculus; Ranunculus glacialis; Rumex; Saxifraga; Saxifraga oppositofolia; Sempervivum tectorum; Silene acaulis; Solanaceae; Soldanella; Trifolium; Trifolium pallescens; Vaccinium uliginosium; Veronica; Viola tricolor; Sediment corer; DNA metabarcoding approach, gh primers
• Online Access: https://dx.doi.org/10.1594/pangaea.958675; https://doi.pangaea.de/10.1594/PANGAEA.958675

This dataset contains plant taxa detected by sedimentary DNA analyses. The dataset is presented in number of DNA sequences by replicate of analyses (12 PCR replicates). It has been generated applying the DNA metabarcoding approach on a 7.75-m long sediment cores retrieved by using an Uwitec piston coring device directly from the ice-covered surface of the lake. The primers used are g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') (Taberlet, P., et al. Power and limitations of the chloroplast trn L (UAA) intron for plant DNA barcoding. Nucleic acids research, 35(3), e14-e14. (2007)). The sequences were then produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNAmetabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of ...