Plant DNA taxa detected in Lake Savine sediments (North-western Alps) over the last 8000 years ...

This dataset contains plant taxa detected by sedimentary DNA analyses. The dataset is presented in number of DNA sequences by replicate of analyses (12 PCR replicates). It has been generated applying the DNA metabarcoding approach on a 7.75-m long sediment cores retrieved by using an Uwitec piston c...

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Bibliographic Details
Main Authors: Giguet-Covex, Charline, Chen, Wentao, Ficetola, Francesco Gentile, Gielly, Ludovic, Pansu, Johan
Format: Dataset
Language:English
Published: PANGAEA 2023
Subjects:
Alpes
Lake sediment core
landscape
plant taxa
Sedimentary DNA
AGE
Alchemilla
Apiaceae
Asteraceae
Asteroideae
Bartsia alpina
Betulaceae
Boraginaceae
Brassicaceae
Campanulaceae
Cardamineae
Carex
Caryophyllaceae
Cerastium cerastoides
Colurieae
Cupressaceae
Euphrasia
Mentheae
Myosotis
Onagraceae
Oxyria digyna
Plantago
Poeae
Potentilla
Primulaceae
Ranunculaceae
Ranunculus
Ranunculus glacialis
Rumex
Saxifraga
Saxifraga oppositofolia
Sempervivum tectorum
Silene acaulis
Solanaceae
Soldanella
Trifolium
Trifolium pallescens
Vaccinium uliginosium
Veronica
Viola tricolor
Sediment corer
DNA metabarcoding approach, gh primers
Online Access:https://dx.doi.org/10.1594/pangaea.958675
https://doi.pangaea.de/10.1594/PANGAEA.958675
Description
Summary:This dataset contains plant taxa detected by sedimentary DNA analyses. The dataset is presented in number of DNA sequences by replicate of analyses (12 PCR replicates). It has been generated applying the DNA metabarcoding approach on a 7.75-m long sediment cores retrieved by using an Uwitec piston coring device directly from the ice-covered surface of the lake. The primers used are g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') (Taberlet, P., et al. Power and limitations of the chloroplast trn L (UAA) intron for plant DNA barcoding. Nucleic acids research, 35(3), e14-e14. (2007)). The sequences were then produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNAmetabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of ...

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