Seawater carbonate chemistry and cellular metabolism of the Arctic copepod Calanus glacialis ...

• Main Authors: Thor, Peter, Vermandele, Fanny, Bailey, Allison, Guscelli, Ella, Loubet‑Sartrou, Léa, Dupont, Sam, Calosi, Piero
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Animalia; Arctic; Arthropoda; Bottles or small containers/Aquaria <20 L; Calanus glacialis; Coast and continental shelf; Laboratory experiment; Other studied parameter or process; Pelagos; Polar; Single species; Zooplankton; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Stage; pH; Metabolite; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Arctic copepod; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.957185; https://doi.pangaea.de/10.1594/PANGAEA.957185

Using a targeted metabolomic approach we investigated the effects of low seawater pH on energy metabolism in two late copepodite stages (CIV and CV) of the keystone Arctic copepod species Calanus glacialis. Exposure to decreasing seawater pH (from 8.0 to 7.0) caused increased ATP, ADP and NAD+ and decreased AMP concentrations in stage CIV, and increased ATP and phospho-L-arginine and decreased AMP concentrations in stage CV. Metabolic pathway enrichment analysis showed enrichment of the TCA cycle and a range of amino acid metabolic pathways in both stages. Concentrations of lactate, malate, fumarate and alpha-ketoglutarate (all involved in the TCA cycle) increased in stage CIV, whereas only alpha-ketoglutarate increased in stage CV. Based on the pattern of concentration changes in glucose, pyruvate, TCA cycle metabolites, and free amino acids, we hypothesise that ocean acidification will lead to a shift in energy production from carbohydrate metabolism in the glycolysis toward amino acid metabolism in the ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-03. ...