Seawater carbonate chemistry and hemocyte physiology in the tanner crab (Chionoecetes bairdi) ...

• Main Authors: Meseck, Shannon, Alix, Jennifer H, Swiney, Katherine M, Long, W Christopher, Wikfors, Gary H, Foy, Robert J
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Chionoecetes bairdi; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Immunology/Self-protection; Laboratory experiment; North Pacific; Single species; Temperate; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; pH; Fluorescence; pH, intracellular; Haemolymph, pH; Hemocyte count; Hemocytes; Salinity; Temperature, water; Carbon, inorganic, dissolved; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification; Tanner crab
• Online Access: https://dx.doi.org/10.1594/pangaea.956752; https://doi.pangaea.de/10.1594/PANGAEA.956752

We used flow cytometry to determine if there would be a difference in hematology, selected immune functions, and hemocyte pH (pHi), under two different, future ocean acidification scenarios (pH = 7.50, 7.80) compared to current conditions (pH = 8.09) for Chionoecetes bairdi, Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water. Total counts of hemocytes did not vary among control and experimental treatments; however, there were significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment. Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment. These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH. Crab hemolymph pH (pHe) averaged 8.09 and did not vary among pH treatments. There was no significant difference in internal pH ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-20. ...