Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ...
Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying o...
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Online Access: | https://dx.doi.org/10.1594/pangaea.956185 https://doi.pangaea.de/10.1594/PANGAEA.956185 |
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ftdatacite:10.1594/pangaea.956185 2023-12-31T10:01:00+01:00 Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... Servetto, Natalia Ruiz, Micaela Belen Martinez, Mariano Harms, Lars de Aranzamendi, M C Alurralde, Gastón Giménez, D Abele, Doris Held, Christoph Sahade, Ricardo José 2023 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.956185 https://doi.pangaea.de/10.1594/PANGAEA.956185 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.956183 https://dx.doi.org/10.1016/0003-2697(76)90527-3 https://dx.doi.org/10.1016/j.marenvres.2013.09.007 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Antarctica apoptosis Caspase Cnemidocarpa verrucosa sp. A laboratory study Potter Cove Tunicata Event label Sample code/label Type of study Date/time start, experiment Date/time end, experiment Species Treatment Temperature, water Sample, wet mass Buffer Sample volume Change of extinction Cnemidocarpa verrucosa, superoxide dismutase, in extract Cnemidocarpa verrucosa, superoxide dismutase, per wet mass Proteins Cnemidocarpa verrucosa, superoxide dismutase, per protein mass Sampling by diver Spectrophotometer UV/Vis, Beckman Coulter, DU800 Calculated average/mean values Calculated Bradford method 1976 Dataset dataset 2023 ftdatacite https://doi.org/10.1594/pangaea.95618510.1594/pangaea.95618310.1016/0003-2697(76)90527-310.1016/j.marenvres.2013.09.007 2023-12-01T11:40:46Z Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Caspases 3/7 activity as indicators of apoptosis intensity was measured using the Caspase-Glow 3/7 Assay kit (Promega, USA) following the manufacturer's instructions. Samples were homogenized (16-33 mg) in lysis buffer consisting in 25 mM HEPES, 5 mM MgCl₂·6H₂O, 1 mM EGTA, 1 μg/mL pepstatin, 1 μg/mL leupectin, and 1 ... Dataset Antarc* Antarctic Antarctica King George Island Ocean acidification Southern Ocean DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Antarctica apoptosis Caspase Cnemidocarpa verrucosa sp. A laboratory study Potter Cove Tunicata Event label Sample code/label Type of study Date/time start, experiment Date/time end, experiment Species Treatment Temperature, water Sample, wet mass Buffer Sample volume Change of extinction Cnemidocarpa verrucosa, superoxide dismutase, in extract Cnemidocarpa verrucosa, superoxide dismutase, per wet mass Proteins Cnemidocarpa verrucosa, superoxide dismutase, per protein mass Sampling by diver Spectrophotometer UV/Vis, Beckman Coulter, DU800 Calculated average/mean values Calculated Bradford method 1976 |
spellingShingle |
Antarctica apoptosis Caspase Cnemidocarpa verrucosa sp. A laboratory study Potter Cove Tunicata Event label Sample code/label Type of study Date/time start, experiment Date/time end, experiment Species Treatment Temperature, water Sample, wet mass Buffer Sample volume Change of extinction Cnemidocarpa verrucosa, superoxide dismutase, in extract Cnemidocarpa verrucosa, superoxide dismutase, per wet mass Proteins Cnemidocarpa verrucosa, superoxide dismutase, per protein mass Sampling by diver Spectrophotometer UV/Vis, Beckman Coulter, DU800 Calculated average/mean values Calculated Bradford method 1976 Servetto, Natalia Ruiz, Micaela Belen Martinez, Mariano Harms, Lars de Aranzamendi, M C Alurralde, Gastón Giménez, D Abele, Doris Held, Christoph Sahade, Ricardo José Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
topic_facet |
Antarctica apoptosis Caspase Cnemidocarpa verrucosa sp. A laboratory study Potter Cove Tunicata Event label Sample code/label Type of study Date/time start, experiment Date/time end, experiment Species Treatment Temperature, water Sample, wet mass Buffer Sample volume Change of extinction Cnemidocarpa verrucosa, superoxide dismutase, in extract Cnemidocarpa verrucosa, superoxide dismutase, per wet mass Proteins Cnemidocarpa verrucosa, superoxide dismutase, per protein mass Sampling by diver Spectrophotometer UV/Vis, Beckman Coulter, DU800 Calculated average/mean values Calculated Bradford method 1976 |
description |
Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Caspases 3/7 activity as indicators of apoptosis intensity was measured using the Caspase-Glow 3/7 Assay kit (Promega, USA) following the manufacturer's instructions. Samples were homogenized (16-33 mg) in lysis buffer consisting in 25 mM HEPES, 5 mM MgCl₂·6H₂O, 1 mM EGTA, 1 μg/mL pepstatin, 1 μg/mL leupectin, and 1 ... |
format |
Dataset |
author |
Servetto, Natalia Ruiz, Micaela Belen Martinez, Mariano Harms, Lars de Aranzamendi, M C Alurralde, Gastón Giménez, D Abele, Doris Held, Christoph Sahade, Ricardo José |
author_facet |
Servetto, Natalia Ruiz, Micaela Belen Martinez, Mariano Harms, Lars de Aranzamendi, M C Alurralde, Gastón Giménez, D Abele, Doris Held, Christoph Sahade, Ricardo José |
author_sort |
Servetto, Natalia |
title |
Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
title_short |
Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
title_full |
Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
title_fullStr |
Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
title_full_unstemmed |
Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ... |
title_sort |
molecular responses of calcifying and non-calcifying antarctic benthic species to ocean acidification - superoxide dismutase activity ... |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://dx.doi.org/10.1594/pangaea.956185 https://doi.pangaea.de/10.1594/PANGAEA.956185 |
genre |
Antarc* Antarctic Antarctica King George Island Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica King George Island Ocean acidification Southern Ocean |
op_relation |
https://dx.doi.org/10.1594/pangaea.956183 https://dx.doi.org/10.1016/0003-2697(76)90527-3 https://dx.doi.org/10.1016/j.marenvres.2013.09.007 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.95618510.1594/pangaea.95618310.1016/0003-2697(76)90527-310.1016/j.marenvres.2013.09.007 |
_version_ |
1786793874814926848 |