Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ...

• Main Authors: Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Antarctica; apoptosis; Caspase; Cnemidocarpa verrucosa sp. A; laboratory study; Potter Cove; Tunicata; Event label; Sample code/label; Type of study; Date/time start, experiment; Date/time end, experiment; Species; Treatment; Temperature, water; Sample, wet mass; Buffer; Sample volume; Change of extinction; Cnemidocarpa verrucosa, superoxide dismutase, in extract; Cnemidocarpa verrucosa, superoxide dismutase, per wet mass; Proteins; Cnemidocarpa verrucosa, superoxide dismutase, per protein mass; Sampling by diver; Spectrophotometer UV/Vis, Beckman Coulter, DU800; Calculated average/mean values; Calculated; Bradford method 1976; Antarc*; Antarctic; King George Island; Ocean acidification; Southern Ocean
• Online Access: https://dx.doi.org/10.1594/pangaea.956185; https://doi.pangaea.de/10.1594/PANGAEA.956185

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Caspases 3/7 activity as indicators of apoptosis intensity was measured using the Caspase-Glow 3/7 Assay kit (Promega, USA) following the manufacturer's instructions. Samples were homogenized (16-33 mg) in lysis buffer consisting in 25 mM HEPES, 5 mM MgCl₂·6H₂O, 1 mM EGTA, 1 μg/mL pepstatin, 1 μg/mL leupectin, and 1 ...