Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity ...

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying o...

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Bibliographic Details
Main Authors: Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José
Format: Dataset
Language:English
Published: PANGAEA 2023
Subjects:
Antarctica
apoptosis
Caspase
Cnemidocarpa verrucosa sp. A
laboratory study
Potter Cove
Tunicata
Event label
Sample code/label
Type of study
Date/time start, experiment
Date/time end, experiment
Species
Treatment
Temperature, water
Sample, wet mass
Buffer
Sample volume
Change of extinction
Cnemidocarpa verrucosa, superoxide dismutase, in extract
Cnemidocarpa verrucosa, superoxide dismutase, per wet mass
Proteins
Cnemidocarpa verrucosa, superoxide dismutase, per protein mass
Sampling by diver
Spectrophotometer UV/Vis, Beckman Coulter, DU800
Calculated average/mean values
Calculated
Bradford method 1976
Antarc*
Antarctic
King George Island
Ocean acidification
Southern Ocean
Online Access:https://dx.doi.org/10.1594/pangaea.956185
https://doi.pangaea.de/10.1594/PANGAEA.956185
Description
Summary:Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Caspases 3/7 activity as indicators of apoptosis intensity was measured using the Caspase-Glow 3/7 Assay kit (Promega, USA) following the manufacturer's instructions. Samples were homogenized (16-33 mg) in lysis buffer consisting in 25 mM HEPES, 5 mM MgCl₂·6H₂O, 1 mM EGTA, 1 μg/mL pepstatin, 1 μg/mL leupectin, and 1 ...

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