Seawater carbonate chemistry in the experiment of transcriptomic responses of coralline algae to global change stressors ...

• Main Authors: Page, Tessa M, McDougall, Carmel, Bar, Ido, Diaz-Pulido, Guillermo
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Gene expression incl. proteomics; Laboratory experiment; Macroalgae; Plantae; Porolithon onkodes; Rhodophyta; Single species; South Pacific; Sporolithon durum; Temperature; Tropical; Type; Treatment; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Carbonate ion; Carbonate ion, standard error; High magnesium calcite saturation state; High magnesium calcite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.955746; https://doi.pangaea.de/10.1594/PANGAEA.955746

Crustose coralline algae (CCA) are calcifying red macroalgae that play important ecological roles including stabilisation of reef frameworks and provision of settlement cues for a range of marine invertebrates. Previous research into the responses of CCA to ocean warming (OW) and ocean acidification (OA) have found magnitude of effect to be species-specific. Response to OW and OA could be linked to divergent underlying molecular processes across species. Here we show Sporolithon durum, a species that exhibits low sensitivity to climate stressors, had little change in metabolic performance and did not significantly alter the expression of any genes when exposed to temperature and pH perturbations. In contrast, Porolithon onkodes, a major coral reef builder, reduced photosynthetic rates and had a labile transcriptomic response with over 400 significantly differentially expressed genes, with differential regulation of genes relating to physiological processes such as carbon acquisition and metabolism. The ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-02-16. ...