Stress biomarkers in the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment in the Azores ...

We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three exper...

Full description

Bibliographic Details
Main Authors: Carreiro-Silva, Marina, Martins, Ines, Raimundo, Joana, Caetano, Miguel, Bettencourt, Raul, Cerqueira, Teresa, Colaço, Ana
Format: Dataset
Language:English
Published: PANGAEA 2022
Subjects:
Cnidaria
enzyme activity
experiment
gene expression
metabolism
metal bioaccumulation
North Atlantic
octocorals
Experimental treatment
Experiment duration
Aquarium number
Specimen identification
Biomarker
Activity description
Spectrophotometer
A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe ATLAS
Integrated Assessment of Atlantic Marine Ecosystems in Space and Time iAtlantic
JPI Oceans - Ecological Aspects of Deep-Sea Mining JPIO-MiningImpact
Managing Impacts of Deep-seA reSource exploitation MIDAS
Online Access:https://dx.doi.org/10.1594/pangaea.948412
https://doi.pangaea.de/10.1594/PANGAEA.948412
Description
Summary:We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three experimental treatments for a period of four weeks at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores): (1) control conditions (no added sediments); (2) suspended polymetallic sulphide (PMS) particles; (3) suspended quartz particles. Antioxidant stress related biomarkers (glutathione S−transferase, superoxide dismutase, catalase, malondialdehyde) in D. aff. meteor tissues were used to evaluate the degree of cellular stress induced by exposure to PMS and quartz particles at times 0 and 13 days in all treatments and from the control and quartz treatments also at time 27 days of the experiment. ... : Cellular stress biomarkers: Results of the enzymatic activities of antioxidant enzymes SOD, CAT, GST and concentration of MDA in D. meteor tissuesMDA: malondialdehyde (nmol/mg of protein,wet weight)SOD: superoxide dismutase (inhibition units/mg of protein, wet weight)CAT: catalase (µmol/min/mg of protein, wet weight).GST: Glutathione S-transferase (nmol/min/mg of protein, wet weight) ...

Similar Items