Seawater carbonate chemistry and whole community and functional gene changes of bioflms on marine plastic debris ...

• Main Authors: Kerfahi, Dorsaf, Harvey, Ben P, Kim, Hyoki, Yang, Ying, Adams, Jonathan M, Hall-Spencer, Jason M
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: CO2 vent; Coast and continental shelf; Community composition and diversity; Entire community; Field observation; Gene expression incl. proteomics; North Pacific; Pelagos; Temperate; Type; Figure; Treatment; Taxa; Abundance; Species; Phylum; Family; Function; Sample ID; Shannon Diversity Index; Functional diversity; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Experiment; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.946567; https://doi.pangaea.de/10.1594/PANGAEA.946567

Plastics are accumulating in the world's oceans, while ocean waters are becoming acidified by increased CO2. We compared metagenome of biofilms on tethered plastic bottles in subtidal waters off Japan naturally enriched in CO2, compared to normal ambient CO2 levels. Extending from an earlier amplicon study of bacteria, we used metagenomics to provide direct insights into changes in the full range of functional genes and the entire taxonomic tree of life in the context of the changing plastisphere. We found changes in the taxonomic community composition of all branches of life. This included a large increase in diatom relative abundance across the treatments but a decrease in diatom diversity. Network complexity among families decreased with acidification, showing overall simplification of biofilm integration. With acidification, there was decreased prevalence of genes associated with cell–cell interactions and antibiotic resistance, decreased detoxification genes, and increased stress tolerance genes. There ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-07-22. ...