Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification ...

We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.268751...

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Bibliographic Details
Main Authors: Lowder, Kaitlyn, deVries, Maya S, Hattingh, Ruan, Day, James M D, Andersson, Andreas J, Zerofski, Phillip, Taylor, Jennifer
Format: Dataset
Language:English
Published: PANGAEA 2022
Subjects:
California Current Ecosystem
crustacean
Laboratory experiment
Ocean acidification
Type of study
Species, unique identification
Species, unique identification URI
Species, unique identification Semantic URI
Specimen identification
Status
Body region
Cuticle layer
Identification
Treatment
Treatment pH
pH, standard deviation
Treatment temperature
Carbon
Nitrogen
Oxygen
Sodium
Magnesium
Aluminium
Silicon
Phosphorus
Sulfur
Chlorine
Calcium
Device type
Quality control
Trap, baited
Scanning electron microscope SEM equipped with electron-dispersive x-ray spectroscopy EDX
Online Access:https://dx.doi.org/10.1594/pangaea.945334
https://doi.pangaea.de/10.1594/PANGAEA.945334
Description
Summary:We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the atomic weight composition (%) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point ...

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