Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi ...

• Main Authors: Zhang, J, Yang, Q, Liu, Q, Liu, S, Zhu, Yue, Yao, Jiang, Wang, Hong, Guan, WanChun
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Karenia mikimotoi; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Single species; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Catalase activity, unit per cell; Catalase activity, standard deviation; Glutathione reductase activity, unit per cell; Glutathione reductase activity, standard deviation; Superoxide dismutase activity, unit per cell; Superoxide dismutase activity, standard deviation; Temperature, water; Salinity; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.945242; https://doi.pangaea.de/10.1594/PANGAEA.945242

The HAB-forming, toxic dinoflagellate Karenia mikimotoi, previously found to benefit from ocean acidification (OA), was cultivated to investigate its transcriptional response to simulated OA for 30 generations. Batch cultures were grown under two CO2 concentrations, 450 (control) and 1100 (simulated OA) μatm, and physiological parameters [growth, pigments, catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) activity], as well as transcriptomes (obtained via RNA-seq), were compared. Chlorophyll a (Chl a) and carotenoid (Caro) contents, as well as CAT and GR activities, were significantly increased under OA conditions. Transcriptomic analysis revealed 2,490 differentially expressed unigenes in response to OA, which comprised 1.54% of all unigenes. A total of 1,121 unigenes were upregulated, and 1,369 unigenes were downregulated in OA compared to control conditions. The downregulated expression of bicarbonate transporter and carbonic anhydrase genes was a landmark of OA acclimation. Key ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-06-08. ...