Seawater carbonate chemistry and the biochemical and transcriptome responses of the clam Ruditapes philippinarum ...

• Main Authors: Almeida, Angela, Freitas, Rosa, Calisto, Vania, Esteves, Valdemar I, Schneider, Rudolf J, Soares, Amadeu M V M, Figueira, Etelvina, Campos, Bruno, Barata, Carlos
• Format: Dataset
• Language: English
• Published: PANGAEA 2018
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Brackish waters; Gene expression incl. proteomics; Laboratory experiment; Mollusca; North Atlantic; Organic toxins; Other metabolic rates; Other studied parameter or process; Ruditapes philippinarum; Single species; Temperate; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Experiment duration; Treatment; Drug concentration per fresh mass; Drug concentration per fresh mass, standard deviation; Bioconcentration factor; Bioconcentration factor, standard deviation; Energy transport system activity, per fresh mass; Electron transport system activity of oyxgen, standard deviation; Lipid peroxidation, per fresh mass; Lipid peroxidation, standard deviation; Total glutathione peroxidase activity, per fresh mass; Total glutathione peroxidase activity, per fresh mass, standard deviation; mRNA levels, relatively to the elongation factor; mRNA levels, relatively to the elongation factor, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.944568; https://doi.pangaea.de/10.1594/PANGAEA.944568

Several works evaluated the toxicity of pharmaceutical drugs and climate related changes in invertebrates but few explored the combined effects of both stressors, namely considering their mode of action (MoA). Carbamazepine (CBZ) and cetirizine (CTZ) are pharmaceutical drugs detected in the environment and the toxicity derived from the combined effects of these drugs with ocean acidification (OA) is poorly explored. Thus, the present study investigated the biochemical parameters related to an oxidative stress response and the transcription of genes related to the MoA of CBZ (1.0 μg/L) and CTZ (0.6 μg/L) in the clam Ruditapes philippinarum chronically exposed (28 days) to control (7.8) and low (7.5) pH conditions. The results obtained showed that despite the clams accumulated both drugs, at low pH the clams exposed to CTZ decreased drug concentration and BCF values (CTZ uptake: 2.0 ± 0.5 ng/g fresh weight; BCF: 3.8 ± 0.9) in comparison with clams exposed to control pH (CTZ uptake: 2.9 ± 0.3 ng/g fresh weight; ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-05-24. ...