Seawater carbonate chemistry and growth rates, carbon utilization and the internal pH regulation at the site of calcification in Emiliania huxleyi, Calcidiscus leptoporus and Pleurochrysis ...

• Main Authors: Liu, Yiwei, Rokitta, Sebastian D, Rost, Björn, Eagle, Robert A
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Acid-base regulation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Pelagos; Phytoplankton; Pleurochrysis carterae; Primary production/Photosynthesis; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; pH; pH, standard deviation; Growth rate; Growth rate, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Carbon, inorganic, particulate, per cell; Particulate inorganic carbon per cell, standard deviation; Particulate organic carbon production per cell; Particulate inorganic carbon production per cell; Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon/particulate organic carbon ratio, standard deviation; δ13C, particulate organic carbon; δ13C, particulate organic carbon, standard deviation; δ13C, particulate inorganic carbon; δ13C, particulate inorganic carbon, standard deviation; δ13C, dissolved inorganic carbon; δ13C, dissolved inorganic carbon, standard deviation; Difference δ13C, particulate organic carbon and dissolved inorganic carbon; Difference δ13C, particulate organic carbon and dissolved inorganic carbon, standard deviation; Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon; Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon, standard deviation; δ11B; δ11B, standard deviation; Calcifying fluid, pH; Calcifying fluid, pH, standard deviation; pH, difference; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.943274; https://doi.pangaea.de/10.1594/PANGAEA.943274

Ocean acidification (OA) appears to have diverse impacts on calcareous coccolithophores, but the cellular processes underlying these responses are not well understood. Here we use stable boron and carbon isotopes, B/Ca ratios, as well as inorganic and organic carbon production rates to investigate the carbon utilization and the internal pH regulation at the site of calcification in Emiliania huxleyi, Pleurochrysis carterae and Calcidiscus leptoporus cultured over a wide pCO2 range (180 to 1000 μatm). Despite large variability, the geochemistry data indicate species-specific modes of pH control and differences in the utilization of inorganic carbon. Boron isotope data suggest that all three species generally upregulate the pH of the calcification fluid (pHCF) compared to surrounding seawater, which coincides with relatively constant growth rates and cellular ratios of inorganic to organic carbon. Furthermore, species exhibit different strategies in regulating their pHCF, i.e., two species maintain homeostasis ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-04-13. ...