Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ...

• Main Authors: Chang, William Weijen, Matt, Ann-Sophie, Schewe, Marcus, Musinszki, Marianne, Grüssel, Sandra, Brandenburg, Jonas, Garfield, David, Bleich, Markus, Baukrowitz, Thomas, Hu, Marian Y
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Animalia; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Echinodermata; Gene expression incl. proteomics; Laboratory experiment; North Atlantic; Pelagos; Single species; Strongylocentrotus purpuratus; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Replicate; Time in days; Gene expression; pH; Carbon, inorganic, dissolved; Alkalinity, total; Partial pressure of carbon dioxide water at sea surface temperature wet air; Temperature, water; Salinity; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.939436; https://doi.pangaea.de/10.1594/PANGAEA.939436

Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification process by providing an exit route for protons liberated by the formation of CaCO3. Using the sea urchin larva, we examined the otopetrin ortholog otop2l, which is exclusively expressed in the calcifying primary mesenchymal cells (PMCs) that generate the calcitic larval skeleton. otop2l expression is stimulated during skeletogenesis, and knockdown of otop2l impairs spicule formation. Intracellular pH measurements demonstrated Zn2+-sensitive H+ fluxes in PMCs that regulate intracellular pH in a Na+/HCO3−-independent manner, while Otop2l knockdown reduced membrane proton permeability. Furthermore, Otop2l displays unique features, including strong activation by high extracellular pH (>8.0) and check-valve–like ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-12-16. ...