Benthic bacterial communities in coastal sandy sediments (Isfjorden, Svalbard) ...
Coastal sands are biocatalytic filters for dissolved and particulate organic matter of marine and terrestrial origin. In this study, we accessed the variability of benthic bacterial communities over two annual cycles in Isfjorden (Svalbard, 78°N) sediments, where primary production does not occur du...
| Main Authors: | , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
PANGAEA
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.1594/pangaea.930483 https://doi.pangaea.de/10.1594/PANGAEA.930483 |
| Summary: | Coastal sands are biocatalytic filters for dissolved and particulate organic matter of marine and terrestrial origin. In this study, we accessed the variability of benthic bacterial communities over two annual cycles in Isfjorden (Svalbard, 78°N) sediments, where primary production does not occur during winter. Sandy sediment samples were obtained with a Van Veen grab. Benthic community structure in surface sediments (0-2 cm depth) was studied on the level of cell counts (DAPI staining, CARD-FISH) and 16S rRNA-based taxonomy (16S rRNA gene sequencing using primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21, Illumina sequencing platform HiSeq2500, 2x250 bases, paired-end). Prior to DAPI staining and CARD-FISH, formaldehyde-fixed Svalbard sediment samples were sonicated on ice with a type MS2.5 probe (Sonoplus mini20; Bandelin, Berlin, Germany). Six sonication steps were done at a setting of 30 s, an amplitude of 86% and pulse of 0.2 s. Combined supernatants were filtered onto 0.2 µm pore size polycarbonate ... |
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